Effect of Temperature on Isolation and Characterization of Hydroxyapatite from Tuna (Thunnus obesus) Bone
نویسندگان
چکیده
The effect of temperature on isolation and characterization of hydroxyapatite (HAp) from tuna bone was evaluated at different temperatures ranging from 200 °C to 1200 °C. The calcined bones were characterized by thermo gravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX) and cytotoxicity assay. The FTIR and TGA results revealed the presence of inorganic and organic matrices in raw bone and a preserved carbonated group in the derived HAp. The XRD results of the derived HAp were coherent with the Joint Committee on Powder Diffraction Standards (JCPDS-09-0432/1996) data. In addition, FE-SEM results revealed the formation of nanostructured HAp (80-300 nm) at 600 °C and crystal agglomeration was observed with an increase in temperature. The calcium to phosphorous weight ratio was determined by EDX results of treated bones. Derived HAp with various crystal sizes had no cytotoxicity on the MG 63 cell line. Based on the analysis, we conclude that varying the isolation temperature between 600-900 °C has tremendous impact on the production of HAp from Thunnus obesus with required properties.
منابع مشابه
Distribution Modeling of Bigeye Tuna (Thunnus obesus Lowe, 1839), Using Satellite Derived Environmental Variables in Indian Ocean
Understanding effects of environment on the distribution of economic fish is a fundamental step in the ecosystem-based management and ultimately a standard approach in management policies. Bigeye tuna (Thunnus obesus) is one of the most important aquatic species harvested in the Indian Ocean. The present study investigated the association of different variables effecting the rate of catch and d...
متن کاملBehaviour of yellowfin (Thunnus albacares) and bigeye tuna (T. obesus) in a network of anchored Fish Aggregation Devices
Behaviour of yellowfin (Thunnus albacares) and bigeye tuna (T. obesus)
متن کاملDifferent residence times of yellowfin tuna, Thunnus albacares, and bigeye tuna, T. obesus, found in mixed aggregations over a seamount
Aggregations of tuna can be found in association with seamounts throughout all the tropical oceans and these aggregations are often exploited by tuna fishing fleets (Fonteneau, 1991). In Hawaii, a fishery has developed over the last decade that targets the mixed species aggregations of predominantly subadult tuna found in association with the Cross Seamount located approximately 160 nmi south o...
متن کاملIdentification of tuna species by a real-time polymerase chain reaction technique
0308-8146/$ see front matter 2012 Elsevier Ltd. A doi:10.1016/j.foodchem.2012.01.076 ⇑ Corresponding author. Tel.: +886 2 33663227; fax E-mail address: [email protected] (J.-C. Shiao). Tuna are highly priced fishes that are often used in processed products. For effective fishery management and protection of consumers’ rights, it is important to develop a molecular method to identify the specie...
متن کاملBigeye Tuna (thunnus Obesus) Behavior and Physiology and Their Relevance to Stock Assessments and Fishery Biology
Bigeye tuna (Thunnus obesus) have distinctive depth distributions and vertical movement patterns. They remain in the uniformed temperature surface layer at night and can descend to greater than 500 m depth at dawn. They thus mirror the vertical migrations of the small nektonic organisms of the deep sound scattering layer and extensively exploit these as a food resource. At their maximum depths,...
متن کامل